| Date | Opposition | H/A | W/L | Score |
| 20/09/09 | Solent & Gosport Devils | A | L | 0-20 |
| 26/09/09 | Streatham Bruins | H | W | 11-3 |
| 10/10/09 | Swindon ENL Wildcats | H | L | 0-8 |
| 18/10/09 | Basingstoke Buffalo | A | L | 1-17 |
| 07/11/09 | Bristol Pitbulls | A | L | 3-7 |
| 28/11/09 | Basingstoke Buffalo | H | L | 1-15 |
| 29/11/09 | Swindon ENL Wildcats | A | L | 1-14 |
| 03/01/10 | Chelmsford Warriors | A | L | 3-10 |
| 09/01/10 | Invicta Mustangs | H | L | 1-14 |
| 23/01/10 | Wightlink Tigers | H | L | 1-8 |
| 30/01/10 | Cardiff ENL Devils | H | L | 0-7 |
| 31/01/10 | Wightlink Tigers | A | L | 2-15 |
| 06/02/10 | Bristol Pitbulls | H | L | 4-21 |
| 07/02/10 | Invicta Dynamos | A | L | 1-14 |
| 13/02/10 | Romford Fury | H | W | 6-4 |
| 20/02/10 | Chelmsford Warriors | H | L | 4-10 |
| 21/02/10 | Romford Fury | A | L | 2-12 |
| 27/02/10 | Solent & Gosport Devils | H | L | 0-7 |
| 20/03/10 | Cardiff ENL Devils | A | L | 2-9 |
| 11/04/10 | Streatham Bruins | A | L | 2-7 |
Match reports for this seaon’s games can be found within the Lions in Print section, all in PDF format.